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In Situ Hybridization (ISH) is a powerful molecular biology technique used for the detection and localization of specific nucleic acid sequences within cells or tissue sections. It utilizes complementary nucleic acid probes labeled with fluorescent or chromogenic tags to bind to target sequences in fixed specimens, enabling visualization and analysis under a microscope. In Situ Hybridization is widely used in cytogenetics, pathology, developmental biology, and neuroscience research for gene expression analysis and genomic mapping. Key specifications include:

• Probe labeling: Fluorescent dyes, chromogenic labels, or hapten molecules for probe visualization
• Hybridization conditions: Stringency control for optimizing probe-target interactions and minimizing non-specific binding
• Detection methods: Fluorescence microscopy, brightfield microscopy, or confocal microscopy for signal detection and imaging
• Sample preparation: Tissue fixation, permeabilization, and pretreatment steps for optimal probe penetration and binding
• Data analysis: Image analysis software for quantification of signal intensity and spatial distribution