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The Thermal Cycler, also known as a PCR machine, is a laboratory instrument used for amplifying specific DNA or RNA sequences through the polymerase chain reaction (PCR). It provides precise thermal cycling conditions to facilitate DNA denaturation, annealing of primers, and extension of DNA strands. The Thermal Cycler is a fundamental tool in molecular biology, genetics, and biotechnology research for applications such as cloning, genotyping, and mutation analysis. Key specifications include:

• Temperature range: Heating and cooling capabilities for rapid and controlled thermal cycling, typically ranging from ambient to 100°C
• Programmability: User-defined temperature profiles for customized PCR protocols and optimization experiments
• Sample capacity: Compatibility with various reaction formats, including PCR tubes, microplates, or slides
• Gradient function: Optional gradient feature for optimizing annealing temperatures across the thermal block
• Touchscreen interface: User-friendly interface for programming, monitoring, and analyzing PCR experiments